This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-terminus region of Human CaM Kinase II protein.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-CaM Kinase II Antibody, Calcium/calmodulin dependent protein kinase II alpha antibody, CaM kinase II alpha antibody, CaM kinase II alpha chain antibody, CaM kinase II alpha subunit antibody, CAMK2A, CAMKA
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:4,000 - 1:20,000, WB: 1:1,000 - 1:3,000
Anwendungsbeschreibung:
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 54 kDa in size corresponding to CaM Kinase II by western blotting in the appropriat
Western blot using Rocklands Affinity Purified anti-CaM Kinase II antibody shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 µg of a mouse brain whole cell lysate (p/n W10-000-T004) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase II was similarly detected on lysates from rat brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
