VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 175-200 of Human VDAC1/Porin1.
Konjugation:
Unconjugated
Alternative Synonym:
Loading Control Antibody, VDAC-1 Antibody, POR1 Antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: User Optimized, IHC: User Optimized, IP: User Optimized, WB: 1:1,000
Anwendungsbeschreibung:
Ready to Use Anti-VDAC/Porin Antibody has been optimized and validated in western blot at 1:1000 dilution. This Anti-VDAC/Porin (RTU) Antibody is sufficient to run 10 western blots. Expect a band approximately 30-33kDa in size corresponding to VDAC/Porin
Western Blot Results of Ready to Use Rabbit Anti-VDAC/Porin Antibody. Lane 1: Opal Pre-stained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: HEK293T WC Lysate (p/n W09-001-GX5). Lane 3: HeLa WC Lysate (p/n W09-000-364). Load: 10µL. Primary Antibody: Ready to Use Rabbit Anti-VDAC1/Porin Antibody 1:1000 overnight at 4C. Secondary Antibody: Goat Anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30 min RT. Block: BlockOut Buffer (p/n MB-073). Expect: ~31 kDa.
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