This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 25-50 of human SPANX protein.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-SPANX antibody, SPANXC, SPANX-C, Sperm protein associated with the nucleus on the X chromosome C, Cancer-testis-associated protein CTp11 antibody, Cancer/testis antigen 11.3, CTP11 antibody, Nuclear associated protein SPAN Xc antibody, SPANX family member C antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:3,000
Anwendungsbeschreibung:
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~97kDa.
Western blot using Rocklands affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells in Lane 1. VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein ~97kDa. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
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