This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 289-300 of human Hsp90 protein.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-Heat Shock Protein HSP 90-alpha antibody, HSP 86 antibody, Heat shock 86kDa antibody, Renal carcinoma antigen NY REN 38 antibody, Heat shock 90kDa protein 1 alpha antibody, Lipopolysaccharide-associated protein 2, LAP-2, HSP90A, HSPC1, HSPCA, HSP90AA1, HSP 90-alpha
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to Hsp90 protein by western blotting in th
Western blot using Rocklands Affinity Purified anti-Hsp90 antibody shows detection of a band at ~90 kDa corresponding to Hsp90 in various lysate preparations (arrowhead). Cos7 transfected with mutant K294R Hsp90 (left two lanes), Cos7 transfected with wild type Hsp90 (middle two lanes) and empty vector Cos7 cells (right two lanes) were either treated with Trichostatin A (an HDAC inhibitor) or mock treated with DMSO only, as indicated. Transfected cells express either mutant or wt Hsp90 coupled to FlagTM tag. Anti-FlagTM immunoprecipitation was performed prior to western blotting with anti-Hsp90. Personal Communication, Brad Scroggins, Urologic Oncology Branch, CCR, NCI, Rockville, MD.
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