Cyclin E2 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Cyclin E2 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-971
• Hersteller Artikelnummer: 600-401-971
• Alternativnummer: ROC-600-401-971
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-Cyclin E2 antibody, G1/S-specific cyclin-E2, CCNE2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.17 mg/mL by UV absorbance at 280 nm
• NCBI: 12448
• UniProt: Q9Z238
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Mouse
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:85,000, IP: 1:100, WB: 1:200 - 1:2,000
• Anwendungsbeschreibung: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to Cyclin E2 protein by western blotti

Bilder

Western blot using Rocklands affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.