This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the carboxy terminus.
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 93 kDa in size corresponding to Cenexin-1 by western blotting in th
Western Blot of Rabbit Anti-Cenexin 1 Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Hu Semen Lysate. Lane 3: MCF7 WCL (p/n W09-000-360). Lane 4: MOLT4 WCL (p/n W09-001-GK2). Lane 5: HeLa WCL (p/n W09-000-364). Load: 10µg/lane. Primary Antibody: Anti-Cenexin-1 at 1.0µg/mL overnight at 2-8C. Secondary Antibody: Goat anti-Rabbit IgG HRP (p/n 611-103-122) at 1:40000 for 30mins at RT. Block: 5% Casein/PBS. Predicted MW: ~95kDa. Observed MW: ~100kDa.
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