This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human KSHV ORF57 protein.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-KSHV ORF57 antibody, MTA, human herpesvirus 8 protein
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band 50-55 kDa in size corresponding to KSHV ORF57 protein by western blotting in the
Western blot using Rocklands affinity purified anti-KSHV ORF57 to detect KSHV ORF57 in HEK293 cells transfected with ORF57 expression vector and ORF57 truncations, or in KSHV infected B-cell line (BCBL-1) treated with or without valproic acid to induce viral replication (arrow). The membrane was probed with the primary antibody diluted 1:7,500 (left) and 1:1,000 (right). Personal Communication, V. Majerciak, M.Zheng, CCR-NCI, Bethesda, MD.
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