This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region human TRAF2.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:60,000 - 1:250,000, IF Microscopy: User Optimized, WB: 1 µg/mL
Anwendungsbeschreibung:
Rabbit Anti-TRAF2 Antibody has been tested in ELISA and Western Blot. Positive control HeLa Whole Cell lysate (p/n W09-000-364) expect ~47kDa. Specific conditions for reactivity should be optimized by the end user.
Western blot using Rocklands affinity purified anti-TRAF2 antibody shows detection of endogenous TRAF2 at ~47kDa (arrowhead). Lane 1 and 3: molecular weight markers. Lane 2: whole HeLa cell lysates (p/n W09-000-364). The identity of lower molecular weight band in lane 2 is unknown. Lane 4: incubated with immunizing peptide. Briefly, each lane contains approximately 14µg of lysate. Membranes were blocked in 3% BSA-TBS 30 min. at room temperature. Primary antibody was used at a 1:500 dilution in 3% BSA-TBS and reacted overnight at 4C. The membrane was washed and reacted with a 1:20,000 dilution conjugated Gt-a-Rabbit DyLight 649 (p/n 611-143-122) for 1 hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers in lanes 1 and 3. Fluorescence image was captured using the VersaDoc Imaging System developed by Bio-Rad.
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