Anti-ICAD antibody was prepared from whole rabbit serum produced by repeated immunizations with a 20 amino acid peptide near the C-terminus of mouse ICAD.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Mouse
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000, IHC: 5µg/mL, IF Microscopy: 5µg/mL, WB: 1:1,000
Anwendungsbeschreibung:
Anti-ICAD Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 37 kDa in Western Blots of specifi
Western Blot of ICAD Antibody. Lane 1: Sample from Mouse Lungs. Lane 2: Sample from Mouse Brain. Lane 3: Sample from Mouse Liver. Lane 4: Sample from Mouse Kidney. Load: 35 µg per lane. Primary antibody: ICAD antibody at 1:1000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 36.6 kDa, 43 kDa for ICAD.
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