Anti-IL-34 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human IL-34 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000 - 1:40,000, WB: 0.25 - 0.5 µg/mL
Anwendungsbeschreibung:
Anti-IL-34 Antibody has been tested for use in ELISA and WB. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~48 kDa in size corresponding to IL-34 by Western blotting in the appropriate cell lysate or extract. De
Western blot of IL-34 Antibody. Lane A: human brain tissue lysate in the absence of blocking peptide. Lane B: human brain tissue lysate in the presence of blocking peptide. Primary antibody: Anti-IL34 at 0.25 µg/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
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