Anti-p90 RSK1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5000 - 1:50,000, WB: 1µg/mL
Anwendungsbeschreibung:
Anti-p90 RSK1 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kDa in size corresponding to p90 RSK1 protein is expected in the
Western Blot Results of Anti-p90 RSK1 antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: A431 WC Lysate (p/n W09-000-361). Lane 3: A431 EGF stimulated (p/n W09-000-362). Lane 4: A431 WC Lysate (p/n W09-000-361). Lane 5: A431 EGF stimulated (p/n W09-000-362). Primary Antibody: Anti-p90 RSK1 at 1µg/mL overnight at 2-8C. Secondary Antibody: Goat anti-Rabbit IgG Peroxidase (p/n 611-103-122) at 1:40,000 for 30min at RT. Blocking Buffer: 5% BLOTTO-TTBS for 30min at RT. Expect: ~82kDa.
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