Anti-Tyrosine Hydroxylase pS40 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser 40 conjugated to KLH.
ELISA: 1:10,000, IHC: 1:1000, IF Microscopy: 1:1000, WB: 1:1000
Anwendungsbeschreibung:
Anti-Tyrosine Hydroxylase pS40 antibody is tested for use in ELISA, Western Blotting, and ICC/IHC and suitable for IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 42/44 kDa in size corresponding
Western Blot of Rabbit anti-Tyrosine Hydroxylase Ser40 antibody. Lane 1: De-Phospho-TH (serial dilution). Lane 2: Phospho-TH (serial dilution). Load: 10 µg per lane. Primary antibody: Tyrosine Hydroxylase pS40 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 60 kDa for Tyrosine Hydroxylase Ser40. Other band(s): none.
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