IRGM Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-600-401-CA8
Artikelname: IRGM Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-600-401-CA8
Hersteller Artikelnummer: 600-401-CA8
Alternativnummer: ROC-600-401-CA8
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Anti-IRGM antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide near the N-terminus of the human IRGM.
Konjugation: Unconjugated
Alternative Synonym: IRGM Antibody, IFI1, IRGM1, LRG47, LRG-47, IFI1, Immunity-related GTPase family M protein, Immunity-related GTPase family M protein 1
Klonalität: Polyclonal
Konzentration: 1.0 mg/ml by UV absorbance at 280 nm
NCBI: 345611
UniProt: A1A4Y4
Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:10,000-1:20,000, IHC: 5 µg/mL, IF Microscopy: 20 µg/mL, WB: 1-2 µg/mL
Anwendungsbeschreibung: Anti-IRGM Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 20 kDa in Western Blots of specifi
Western Blot of IRGM antibody. Lane A: Rat brain lysate at 1 µg/mL. Lane B: Rat brain lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 20.1 kDa, ~32kDa for IRGM.