Anti-JMJD3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the N-terminus of human JMJD3.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5,000, IF Microscopy: 5 µg/mL, WB: 0.5 µg/mL
Anwendungsbeschreibung:
Anti-JMJD3 Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 177 kDa in Western Blots of specifi
Immunocytochemistry of JMJD3. Cell: K562 Cells. Fixation: fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen Retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-JMJD3 antibody at 2.5 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.
Immunofluorescence of JMJD3. Cell: K562 Cells. Fixation: 4% paraformaldehyde-fixed. Primary Antibody: JMJD3 at 20 µg/mL. Sencondary: goat anti-rabbit IgG antibody at 1:500 dilution (red).
Western Blot Validation of JMJD3. Load: 15µg of Human, Mouse, or Rat Cell Line lysates per lane. Primary Antibody: JMJD3 at 1 µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot of JMJD3. Load: 15 µg of lysates per lane. Primary Antibody: Top: JMJD3 (p/n 600-401-CB8) at 1 µg/mL, Middle: JMJD3 at 2 µg/mL, and Bottom: beta-actin at 1 µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot of JMJD3. Load: 15 µg of K562 Cell Lysate per lane. Lane 1: in the absence of blocking peptide, Lane 2: in the presence of blocking peptide. Primary Antibody: JMJD3 at 0.5 µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot of JMJD3. Load: 15 µg of mouse A-20 lysates per lane. Lane 1: in the absence of blocking peptide, Lane 2: in the presence of blocking peptide. Primary Antibody: JMJD3 at 0.5 µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot of JMJD3. Load: 15 µg of HepG2 lysates per lane. Primary Antibody: JMJD3 at (Lane 1: 0.25µg/mL in the absence of blocking peptide, Lane 2: 0.5µg/mL in the absence of blocking peptide, and Lane 3: 0.25µg/mL in the presence of blocking peptide) for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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