MED28 Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-600-401-CT1
Artikelname: MED28 Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-600-401-CT1
Hersteller Artikelnummer: 600-401-CT1
Alternativnummer: ROC-600-401-CT1
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC, WB
Spezies Reaktivität: Human, Mouse
Immunogen: Anti-MED28 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide from near the internal region of human MED28.
Konjugation: Unconjugated
Alternative Synonym: MED28 Antibody, EG1, magicin, 1500003D12Rik, EG1, FKSG20, Mediator of RNA polymerase II transcription subunit 28, Endothelial-derived protein 1, Magicin
Klonalität: Polyclonal
Konzentration: 1 mg/mL by UV absorbance at 280 nm
NCBI: 80306
UniProt: Q9H204
Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:10,000, IHC: 2.5 µg/mL, IF Microscopy: 20 µg/mL, WB: 1 µg/mL
Anwendungsbeschreibung: Anti-MED28 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 20 kDa in Western Blots of specifi
Western Blot of MED28 antibody. Lane A: Human brain tissue lysate in the absence of blocking peptide. Lane B: Human brain tissue lysate in the presence of blocking peptide. Load: 35 µg per lane. Primary Antibody: Anti-MED28 at 1 µg/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 19.5 kDa, ~19 kDa for MED28.