14-3-3 phospho S58 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: 14-3-3 phospho S58 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-D02
• Hersteller Artikelnummer: 600-401-D02
• Alternativnummer: ROC-600-401-D02
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IHC, WB
• Spezies Reaktivität: Human, Rat
• Immunogen: Anti-14-3-3 pS58 antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58.
• Konjugation: Unconjugated
• Alternative Synonym: 14-3-3 protein beta/alpha, Prepronerve growth factor RNH-1, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/alpha N-terminally processed, Ywhab

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: titrated reagent Sufficient to run approximately 10 miniblots
• NCBI: 56011
• UniProt: P35213
• Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Formulierung: Liquid
• Target-Kategorie: Rat
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000, IHC: 1:500, WB: 1:1000
• Anwendungsbeschreibung: Anti-14-3-3 pS58 antibody is tested for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to the 14-3-3 proteins phosphorylated at S

Bilder

Western Blot of Rabbit Anti-14-3-3 pS58 Antibody. Lane 1: rat brainstem lysate. Lane 2: rat brainstem lysate blocked by the phosphopeptide. Load: 10 µg per lane. Primary antibody: 14-3-3 pS58 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~29kDa, ~29kDa for 14-3-3 protein phosphorylated at Ser58. Other band(s): none.