EphrinB phospho Y317 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: EphrinB phospho Y317 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-D36
• Hersteller Artikelnummer: 600-401-D36
• Alternativnummer: ROC-600-401-D36
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Mouse, Rat
• Immunogen: Anti-EphrinB pY317 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr317 of Xenopus EphrinB conjugated to KLH.
• Konjugation: Unconjugated
• Alternative Synonym: Ephrin type-B receptor 2, EPH-like kinase 5, EK5

Produkteigenschaften

• Klonalität: Polyclonal
• NCBI: 396513
• UniProt: P28693
• Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Formulierung: Liquid
• Target-Kategorie: Chicken
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000, WB: 1:1000
• Anwendungsbeschreibung: Anti-EphrinB pY317 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 46 kDa in size corresponding to EphrinB protein phosphorylated at Tyr317 i

Bilder

Western Blot of Anti-EphrinB pT317 (Rabbit) Antibody. Lane 1: rat testes. Lane 2: rat testes incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: EphrinB pT317 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~46kDa/~46kDa for EphrinB phosphorylated at Tyr317.