MEK1 phospho T292 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: MEK1 phospho T292 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-D72
• Hersteller Artikelnummer: 600-401-D72
• Alternativnummer: ROC-600-401-D72
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Human
• Immunogen: Anti-MEK1 pT292 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr292 conjugated to KLH.
• Konjugation: Unconjugated
• Alternative Synonym: Dual specificity mitogen-activated protein kinase kinase 1, MAPKK 1, ERK activator kinase 1,MAPK/ERK kinase 1

Produkteigenschaften

• Klonalität: Polyclonal
• NCBI: 5604
• UniProt: Q02750
• Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Formulierung: Liquid
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB: 1:1000
• Anwendungsbeschreibung: Anti-MEK1 pT292 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 45 kDa in size corresponding to MEK 1 protein phosphorylated at Thr292 in the

Bilder

Western Blot of Rabbit anti-MEK1 pT292 antibody. Lane 1: recombinant MEK 1 wild type. Lane 2: recombinant MEK 1 wild type with MAP kinase. Lane 3: mutant MEK 1. Lane 4: mutant MEK 1 with MAP kinase. Load: 10 µg per lane. Primary antibody: MEK1 pT292 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 45 kDa, ~45 kDa for MEK1 pT292. Other band(s): none.