MEK1 phospho T386 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: MEK1 phospho T386 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-D73
• Hersteller Artikelnummer: 600-401-D73
• Alternativnummer: ROC-600-401-D73
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Human, Rat
• Immunogen: Anti-MEK1 pT386 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr 386 conjugated to KLH.
• Konjugation: Unconjugated
• Alternative Synonym: Dual specificity mitogen-activated protein kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1

Produkteigenschaften

• Klonalität: Polyclonal
• NCBI: 5604
• UniProt: Q02750
• Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Formulierung: Liquid
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB: 1:1000
• Anwendungsbeschreibung: Anti-MEK1 pT386 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 45 kDa in size corresponding to the MEK1 protein phosphorylated at Thr386 in t

Bilder

Western Blot of Rabbit Anti-MEK1 pT386 antibody. Lane 1: human T47D cells. Lane 2: human T47D cells incubated in lambda Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: MEK1 pT386 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 45 kDa, 45 kDa for MEK 1. Other band(s): none.