Anti-mGluR1a Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.
Anti-mGluR1a antibody is tested for use in Western Blotting and IHC. Expect a band of approximately 125 kDa monomer and 250 kDa mGluR1a dimer in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are
Western Blot of Rabbit Anti-metabotropic glutamate receptors (mGluR) 1a antibody. Lane 1: HEK 293 cells expressing mGluR5. Lane 2: HEK 293 cells expressing mGluR1a. Load: 10 µg per lane. Primary antibody: mGluR1a antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~125k monomer and the ~250k dimer of mGluR1a. Other band(s): No reactivity toward mGluR5.
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