Anti-Nhe-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide near the internal region of the human Nhe-1.
Anti-Nhe-1 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 91 kDa in Western Blots of specifi
Immunofluorescence Microscopy of Nhe-1 antibody. Tissue: Human brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Nhe-1 antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Nhe-1 as a red fluorescent signal.
Western Blot of Nhe-1 antibody. Lane A: MOLT4 cell lysate at 1 µg/mL. Lane B: MOLT4 cell lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 90.7 & 61 kDa, ~90 & ~48 kDa for Nhe-1.
Immunohistochemistry of Nhe-1 antibody. Tissue: Human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Nhe-1 antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Nhe-1 is nuclear and occasionally cytoplasmic. Staining: Nhe-1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Immunohistochemistry of Nhe-1. Tissue: Human Small Intestine. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen Retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-Nhe-1 antibody at 2 µg/ml overnight at 4C. Secondary antibody: A goat anti-rabbit IgG H&L (HRP) at 1:250 dilution. Counter stained with Hematoxylin.
Western Blot of Nhe1. Load: 10 µg of K562 Cell lysate. Primary Antibody: Nhe-1 at 4 µg/mL for 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry of Nhe-1. Tissue: Mouse Colon. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen Retrieval: heat mediation with a citrate buffer (pH6).Primary Antibody: anti-Nhe-1 antibody at 1 µg/ml overnight at 4C. Secondary Antibody: goat anti-rabbit IgG H&L (HRP) at 1:250 dilution. Counter stained with Hematoxylin.
Western Blot of Nhe1. Load: 15 µg of Mouse Tissue lysates per lane. Primary Antibody: Nhe-1 at 1 µg/mL for 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry of Nhe-1. Tissue: Rat Small Intestine. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen Retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-Nhe-1 antibody at 1 µg/ml overnight at 4C. Secondary Antibody: goat anti-rabbit IgG H&L (HRP) at 1:250 dilution. Counter stained with Hematoxylin.
Western Blot of Nhe1. Load: 15 µg of rat brain lysate. Primary Antibody: Nhe-1 at 1 µg/mL for 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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