PKR Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: PKR Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-DP7
• Hersteller Artikelnummer: 600-401-DP7
• Alternativnummer: ROC-600-401-DP7
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Anti-PKR antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to 14 amino acids near the C-terminus of human PKR. The immunogen is located within the last 50 amino acids of PKR.
• Konjugation: Unconjugated
• Alternative Synonym: PKR Antibody, PKR, PRKR, EIF2AK1, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 5610
• UniProt: P19525
• Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:5,000-1:20,000, IHC: 2.5-5 µg/mL, IF Microscopy: 20 µg/mL, WB: 0.5-1 µg/mL
• Anwendungsbeschreibung: Anti-PKR Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 62 kDa in Western Blots of specific

Bilder

Immunohistochemistry of Rabbit Anti-PKR Antibody. Tissue: Mouse Lung. Fixation: paraffin-embedded formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-PKR antibody at 5µg/ml overnight at 4C. Secondary Antibody: goat anti-rabbit IgG H&L (HRP) at 1/250. Counter stained with Hematoxylin.

Immunohistochemistry of PKR antibody. Tissue: rat lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: PKR antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: PKR is nuclear and cytoplasmic. Staining: PKR is stained with toluidine blue.

Immunofluorescence Microscopy of PKR antibody. Cell Type: rat lung cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: PKR antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: PKR is nuclear and cytoplasmic. Staining: PKR as red fluorescent signal.

Immunofluorescence of Rabbit Anti-PKR Antibody. Tissue: Mouse Lung. Fixation: 4% paraformaldehyde-fixed Primary Antibody: PKR at 20µg/mL, Secondary Antibody: goat anti-rabbit IgG antibody at 1/500 dilution (red) and DAPI staining (blue).

Western Blot of PKR antibody in A431 whole cell lysate. Lane A: PKR antibody at 0.5 µg/mL. Lane B: PKR antibody at 1 µg/mL. Lane C: PKR antibody at 2 µg/mL. Load: 35 µg per lane. Primary antibody: PKR antibody at designated concentrations for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 62 kDa, 72 kDa for PKR.

Western Blot of Rabbit Anti-PKR Antibody. Lysates: Human Cell Lines. Loading: 15µg of lysates per lane. Primary Antibody: PKR (1µg/mL), 1hr at RT in 5% NFDM/TBST. Secondary Antibody: Goat anti-rabbit IgG HRP conjugate at 1:10,000. Expect: ~62kDa.

Western Blot of Anti-PKR Antibody. Loading: 15 µg of lysates per lane. Primary Antibodies: PKR (p/n 600-401-DP7) (1 µg/mL), PKR (p/n 600-401-DP8) (1 µg/mL), and beta-actin (1 µg/mL), 1hr at RT in 5% NFDM/TBST. Secondary Antibody: Goat anti-rabbit IgG HRP conjugate at 1:10,000. Expect: ~62kDa.