Anti-Tryptophan Hydroxylase pS260 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 of Tryptophan Hydroxylase.
Anti-Tryptophan Hydroxylase pS260 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of 55 kDa in size corresponding to tryptophan hydroxylase protein phosphoryla
Western Blot of Rabbit anti-Tryptophan Hydroxylase pS260 antibody. Lane 1: Rat Brainstem Lysate (Control). Lane 2: Phospho-Peptide. Lane 3: Non-Phospho Peptide. Load: 10 µg per lane. Primary antibody: Tryptophan Hydroxylase pS260 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 55 kDa for Tryptophan Hydroxylase pS260. Other band(s): Tryptophan Hydroxylase pS260 splice variants and isoforms.
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