Anti-Tyrosine Hydroxylase pS31 Antibody was produced repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser31.
Anti-Tyrosine Hydroxylase pS31 (Rabbit) antibody is tested for use in Western Blotting, IHC and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 60 kDa in size corresponding to tyrosine hydroxyla
Western Blot of Rabbit anti-Tyrosine Hydroxylase pS31 antibody. Lane 1: PC-12 cells incubated in the absence of okadaic acid (Control). Lane 2: PC-12 cells incubated in the presence of okadaic acid (OA, 1 µM for 60 min). Load: 10 µg per lane. Primary antibody: Tyrosine Hydroxylase pS31 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 60 kDa for Tyrosine Hydroxylase pS31. Other band(s): none.
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