Anti-RPS6K1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the C-terminus of human RPS6K1.
Konjugation:
Unconjugated
Alternative Synonym:
RPS6K1 Antibody, RSK, HU-1, RSK1, MAPKAPK1A, Ribosomal protein S6 kinase alpha-1, 90 kDa ribosomal protein S6 kinase 1, S6K-alpha-1
Anti-RPS6K1 Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 83 kDa in Western Blots of specifi
Immunohistochemistry of SAPAP2 antibody. Tissue: Human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: SAPAP2 antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: SAPAP2 is nuclear and occasionally cytoplasmic. Staining: SAPAP2 as a precipitated red signal with hematoxylin purple nuclear counterstain.
Immunofluorescence Microscopy of SAPAP2 antibody. Tissue: Human brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: SAPAP2 at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: SAPAP2 as a red fluorescent signal.
Western Blot of SAPAP2antibody. Lane A: L1210 cell lysate at 0.5 µg/mL. Lane B: L1210 cell lysate at 1 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 82.7 kDa, ~116 kDa for SAPAP2. Other band(s): SAPAP2 splice variants and isoforms.
Immunofluorescence Microscopy of RP S6 Kinase antibody. Cell Type: Jurkat cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: RP S6 Kinase antibodyat 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: RP S6 Kinase is nuclear and cytoplasmic. Staining: RP S6 Kinase as red fluorescent signal.
Western Blot ofRPS6K1 antibody in Jurkat cell lysate. Lane A: RPS6K1 antibody at 2.5 µg/mL. Lane B: RPS6K1 antibody at 5 µg/mL. Lane C: RPS6K1 antibody at 10 µg/mL. Load: 35 µg per lane. Primary antibody: RPS6K1 antibody at designated concentrations for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 82 kDa, 72 kDa for RPS6K1. Other band(s): RPS6K1 splice variants and isoforms.
Immunocytochemistry of RPS6K1 antibody. Cell Type: Jurkat cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: RPS6K1 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: RPS6K1 is nuclear and cytoplasmic. Staining: RPS6K1 is stained with hematoxylin purple nuclear counterstain.
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