Anti-SKA1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 13 amino acid synthetic peptide from near the C-terminus of human SKA1.
Konjugation:
Unconjugated
Alternative Synonym:
SKA1 Antibody, C18orf24, C18orf24, Spindle and kinetochore-associated protein 1
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5,000 - 1:10,000, WB: 0.5-1 µg/mL
Anwendungsbeschreibung:
Anti-SKA1 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 29 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of SKA1 antibody. Lane 1: A549 cell lysate with SKA1 antibody at 0.5 µg/mL. Lane 2: A549 cell lysate with SKA1 antibody at 1 µg/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 29 kDa, 32 kDa for SKA1. Other band(s): SKA1 splice variants and isoforms.
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