HO-1 Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-600-401-F48
Artikelname: HO-1 Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-600-401-F48
Hersteller Artikelnummer: 600-401-F48
Alternativnummer: ROC-600-401-F48
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Canine, Human, Mouse, Rat
Immunogen: HO-1 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against a human heme-oxygenase (HO-1) synthetic multiple antigenic synthetic peptide.
Konjugation: Unconjugated
Alternative Synonym: Hsp32, HMOX1, Heme oxygenase 1, HO, HO1
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
NCBI: 3162
UniProt: P09601
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: IHC: User Optimized, IF Microscopy: 1:100, WB: 1:1000
Anwendungsbeschreibung: Anti-HO-1 Antibody has been tested in WB, IHC, IF. Expect a band approximately ~32kDa protein, corresponding to the molecular mass of HO-1 on SDS Page immunoblots. Specific conditions for reactivity should be optimized by the end user.
Western Blot of rabbit anti-HO-1 antibody. Lane 1: Human cell line mix. Lane 2: none. Load: 35 µg per lane. Primary antibody: HO-1 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 32.8 kDa, ~35 kDa for HO-1. Other band(s): none.