HO-1 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against a human heme-oxygenase (HO-1) synthetic multiple antigenic synthetic peptide.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
IHC: User Optimized, IF Microscopy: 1:100, WB: 1:1000
Anwendungsbeschreibung:
Anti-HO-1 Antibody has been tested in WB, IHC, IF. Expect a band approximately ~32kDa protein, corresponding to the molecular mass of HO-1 on SDS Page immunoblots. Specific conditions for reactivity should be optimized by the end user.
Western Blot of rabbit anti-HO-1 antibody. Lane 1: Human cell line mix. Lane 2: none. Load: 35 µg per lane. Primary antibody: HO-1 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 32.8 kDa, ~35 kDa for HO-1. Other band(s): none.
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