Anti-TIM-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the internal region of human TIM-1.
Konjugation:
Unconjugated
Alternative Synonym:
TIM-1 Antibody, TIM, KIM1, TIM1, HAVCR, KIM-1, TIMD1, TIMD-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, HAVcr-1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell immunoglobulin mucin receptor 1, T-cell membrane protein 1, CD365
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000 - 1:20,000, WB: 1-2 µg/mL
Anwendungsbeschreibung:
Anti-TIM-1 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 39 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of TIM-1 antibody in human uterus tissue lysate. Lane A: TIM-1 antibody at 1 µg/mL. Lane B: TIM-1 antibody at 2 µg/mL. Load: 35 µg per lane. Primary antibody: TIM-1 antibody at designated concentrations for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 75 kDa, 49 kDa for TIM-1. Other band(s): TIM-1 splice variants and isoforms.
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