VENTX Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: VENTX Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-FW2
• Hersteller Artikelnummer: 600-401-FW2
• Alternativnummer: ROC-600-401-FW2
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Anti-VENTX antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide near the internal region of human VENTX.
• Konjugation: Unconjugated
• Alternative Synonym: VENTX Antibody, NA88A, HPX42B, VENTX2, Homeobox protein VENTX, VENT homeobox homolog

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 27287
• UniProt: O95231
• Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000 - 1:20,000, IHC: 5 µg/mL, IF Microscopy: 20 µg/mL, WB: 1-2 µg/mL
• Anwendungsbeschreibung: Anti-VENTX Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 28 kDa in Western Blots of specifi

Bilder

Immunohistochemistry of VENTX antibody. Tissue: Rat brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: VENTX antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: VENTX is cytoplasmic. Staining: VENTX as precipitated pink signal with blue nuclear counterstain.

Immunofluorescence Microscopy of VENTX antibody. Tissue: Rat brain tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: VENTX antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: VENTX is cytoplasmic. Staining: VENTX as red fluorescent signal.

Western Blot of VENTX antibody. Lane 1: Mouse brain tissue lysate with VENTX antibody at 1 µg/mL. Lane 2: Mouse brain tissue lysate with VENTX antibody at 2 µg/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 28 kDa, 31 kDa for VENTX. Other band(s): VENTX splice variants and isoforms.