Vinculin Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Vinculin Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-FW4
• Hersteller Artikelnummer: 600-401-FW4
• Alternativnummer: ROC-600-401-FW4
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Anti-Vinculin antibody was prepared from whole rabbit serum produced by repeated immunizations with a 23 amino acid peptide near the internal region of human Vinculin.
• Konjugation: Unconjugated
• Alternative Synonym: Vinculin Antibody, MV, MVCL, CMD1W, CMH15, HEL114, Vinculin, Metavinculin, MV

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 7414
• UniProt: P18206
• Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:5000 - 1:10000, IF Microscopy: 20 µg/mL, WB: 0.5-1 µg/mL
• Anwendungsbeschreibung: Anti-Vinculin Antibody has been tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 124 kDa in Western Blots of spe

Bilder

Western Blot of Rabbit anti-VINCULIN antibody. Lane 1: A431 WCL. Lane 2: Daudi WCL. Lane 3: Jurkat WCL. Lane 4: Raji WCL. Lane 5: THP-1 whole cell lysate. Primary antibody: VINCULIN antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Goat anti-rabbit HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 120 kDa, 117 kDa for VINCULIN.