Anti-VPS53 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 18 amino acid synthetic peptide from near the internal region of human VPS53.
Konjugation:
Unconjugated
Alternative Synonym:
VPS53 Antibody, HCCS1, hVps53L, pp13624, Vacuolar protein sorting-associated protein 53 homolog
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5000 - 1:10000, WB: 0.5-1 µg/mL
Anwendungsbeschreibung:
Anti-VPS53 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 80 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of VPS53 antibody. Lane A: 293 cell lysate at 0.5 µg/mL. Lane B: 293 cell lysate at 1 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 56.5 kDa, ~100 kDa for VPS53.
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