Anti-GAB1 pY656 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y659 of human GAB1 protein.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-GAB1 pY659 antibody, GAB-1, GAB 1, GRB2-associated-binding protein 1, GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:2,000 - 1:10,000, WB: 1:500-1:2000
Anwendungsbeschreibung:
Anti-GAB1 pY656 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at 76.6 kDa by western blotting in the appropriate cell lysate or extract. Th
Western Blot of Rabbit anti-GAB1 pY659 antibody. Load: Phospho GAB1 rProtein 0.05 µg per lane. Blot Lane 1: GAB1 pY659 primary antibody at 1µg/mL in absence of blocking peptide overnight at 4C. Blot Lane 2: GAB1 pY659 primary antibody at 1µg/mL incubated with 50µg of GAB1 pY659 peptide overnight at 4C. Secondary antibody: HRP Goat Anti-Rabbit IgG secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) Fluorescent blocking buffer for 30 min at RT. Predicted/Observed size: 130 kDa, 130 kDa for GAB1pY659. Other band(s): none.
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