Anti-RON pY1353 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1353 of human RON protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 5.0 µg/ml, WB: 1.0 ug/ml
Anwendungsbeschreibung:
Anti-RONpY1353 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 152.3 kDa corresponding to full length protein by western blotting in the
Western Blot Results of Rabbit Anti-RONpY1353 Antibody. Lane 1: Opal Prestained Ladder - (p/n MB-210-0500). Lane 2: rRON-GST. Lane 3: rPhospho-RON-GST. Lane 4: GST alone (p/n 000-001-200). Load: 50ng. Primary Antibody: Anti-RONpY1353 at 1ug/mL overnight at 4C. Secondary Antibody: Goat anti-Rabbit HRP conjugated - (p/n 611-103-122) at 1:70,000. Block: Blocking Buffer (p/n MB-070) for 30min at RT.
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