RON phospho Y1360 Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-600-401-G70
Artikelname: RON phospho Y1360 Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-600-401-G70
Hersteller Artikelnummer: 600-401-G70
Alternativnummer: ROC-600-401-G70
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Anti-RON pY1360 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1360 of human RON protein.
Konjugation: Unconjugated
Alternative Synonym: rabbit anti-RON pY1360 antibody, Macrophage-stimulating protein receptor, MSP receptor, Protein-tyrosine kinase 8, p185-Ron, PTK8, RON, c-met-related tyrosine kinase, CD136
Klonalität: Polyclonal
Konzentration: 0.79 mg/mL by UV absorbance at 280 nm
NCBI: 4486
UniProt: Q04912
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:2,000 - 1:10,000, WB: 1:500-1:2000
Anwendungsbeschreibung: Anti-RONpY1360 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 152.3 kDa corresponding to full length protein by western blotting in the
Western Blot of Rabbit anti-RONpY1360 antibody. Lane 1: Phospho RON rProtein (cytoplasmic domain). Lane 2: Phospho RON rProtein (cytoplasmic domain) incubated with RONpY1360 peptide. Load: 0.05 µg per lane. Primary antibody: RON pY1360 antibody at 1µg for overnight at 4C. Secondary antibody: HRP Goat anti-rabbit IgG secondary antibody (p/n 611-103-122) at 1:40,000 for 45 min at RT. Block: Fluorescent blocking buffer (p/n MB-070) overnight at 4C. Predicted/Observed size: 152 kDa (full length) /~75 kDa (cytoplasmic domain) RON pY1360. Other band(s): none.