ZIP7 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ZIP7 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-GF2
• Hersteller Artikelnummer: 600-401-GF2
• Alternativnummer: ROC-600-401-GF2
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Anti-ZIP7 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide near the N-terminus of human ZIP7.
• Konjugation: Unconjugated
• Alternative Synonym: ZIP7 Antibody, KE4, HKE4, ZIP7, RING5, H2-KE4, D6S115E, D6S2244E, Zinc transporter SLC39A7, Histidine-rich membrane protein Ke4

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 7922
• UniProt: Q92504
• Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:5000 - 1:10000, IHC: 2.5 µg/mL, IF Microscopy: 20 µg/mL, WB: 0.5-1 µg/mL
• Anwendungsbeschreibung: Anti-ZIP7 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 50 kDa in Western Blots of specific

Bilder

Immunofluorescence Validation of ZIP7. Tissue: Human Brain Tissue. Fixation: 4% paraformaldehyde-fixed. Primary Antibody: Anti-ZIP7 at 20 µg/mLSecondary Antibody: goat anti-rabbit IgG antibody at 1:500 dilution (red).

Immunohistochemistry Validation of ZIP7. Tissue: Human Brain Tissue. Fixation: paraffin-embedded, formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-ZIP7 antibody (6093) at 2.5 µg/ml overnight at 4C. Secondary Antibody: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.

Independent Antibody Validation of ZIP7 via Protein Expression. Loading: 15 µg of human cell lysates per lane. Lane 1: 293, Lane 2: A549, Lane 3: HeLa, Lane 4: HepG2, Lane 5: K562, Lane 6: MCF-7, Lane 7: SK-N-SH. Primary antibody: Anti-ZIP7 (p/n 600-401-GF2 top blot) at 1µg/mL, anti-ZIP7 (middle blot) at 4 µg/mL, and anti-beta-actin (bottom blot) at 1 µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot of ZIP7.Loading: 15 µg of human cell lysates per lane. Lane 1: 293, Lane 2: A549, Lane 3: HeLa, Lane 4: HepG2, Lane 5: K562, Lane 6: MCF7, Lane 7: SK-N-SH. Primary antibody: Anti-ZIP7 at 1µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot of ZIP7. Loading: 15 µg of lysates per lane. Lane 1: Human Brain Tissue, Lane 2: 3T3 (Balb) Cell Lysate. Primary antibody: Anti-ZIP7 1µg/mL at 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot of ZIP7.Loading: 15 µg of Mouse Brain Tissue lysates per lane. Primary antibody: Anti-ZIP7 at (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL) for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot of ZIP7. Loading: 15 µg of rat brain lysates per lane. Primary antibody: Anti-ZIP7 at (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL) for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.