ERK1 N-Term Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ERK1 N-Term Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-GP0
• Hersteller Artikelnummer: 600-401-GP0
• Alternativnummer: ROC-600-401-GP0
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Anti-ERK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-ERK-1 Antibody, MAPK3, ERK 1, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, microtubule-associated protein 2 kinase, p44-ERK1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 27361
• UniProt: P27361
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 5.0 ug/mL, WB: 1.0 ug/mL
• Anwendungsbeschreibung: Anti-ERK 1 (RABBIT) antibody has been tested in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43 kDa.

Bilder

Western Blot of Rabbit Anti-ERK1 N-Term Antibody. Lane 1: Molecular weight marker. Lane 2: ERK1 double mutant WC Lysate. Lane 3: ERK2 protein. Lane 4: Mouse Brain WC Lysate (p/n W10-000-T004). Lane 5: HEK293 WC Lysate (p/n W09-000-365). Load: 10ng lysate or 50ng protein. Blocking: Universal Blocking Buffer (p/n MB-073) for 30 min RT. Primary antibody: Anti-ERK1 N-Term at 1µg/mL in MB-073overnight at 4C. Secondary antibody: Goat anti-Rabbit HRP (p/n 611-103-122) 1:40,000 in buffer (p/n MB-073) for 30 min at RT. Expect: ~43kDa.