Pax7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Pax7 protein.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-Pax7 antibody, Paired box protein Pax-7, Pax 7, Pax-7
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Mouse
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000-1:40:000, WB: 1.0 ug/ml
Anwendungsbeschreibung:
Anti-Pax7 antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 54.9 kDa in size corresponding to Pax7 by western blotting in the appropriate cell lysate or
Western Blot of Rabbit anti-Pax7 antibody. Lane 1: Opal Pre-stained ladder (p/n MB-210-0500). Lane 2: Mouse muscle protein lysate. Lane 3: C2C12 Lysate (p/n W09-001-GL7). Lane 4: Ms spleen lysate (p/n W10-000-T029). Lane 5: Ms brain lysate (p/n W10-000-T004). Lane 6: HEK293T Lysate (W09-001-GX5). Lane 7: HeLa WCL (p/n W09-000-364). Lane 8: MCF7 Lysate (p/n W09-000-360). Lane 9: Jurkat Lysate (p/n W09-001-370). Lane 10: A431 Lysate (p/n W09-000-361). Lane 11: A549 Lysate (p/n W09-001-372). Lane 12: LNCap Lysate (p/n W09-001-GJ9). Lane 13: MOLT-4 Lysate (p/n W09-001-GK2). Lane 14: Ramos Lysate (p/n W09-001-369). Lane 15: Raji Lysate (W09-001-368). Lane 16: Opal Pre-stained ladder (p/n MB-210-0500). Load: 10 µg per lane. Primary antibody: Pax7 antibody at 1:1,000 for overnight at RT. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: ~55 kDa for Pax7.
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