Anti-ASAP1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-terminus region of human ASAP1 protein.
Konjugation:
Unconjugated
Alternative Synonym:
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1, 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein, ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, DEF-1, Differentiation-enhancing factor 1, PIP2-dependent ARF1 GAP
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:50,000, WB: 1µg/mL
Anwendungsbeschreibung:
Anti-ASAP1 Antibody has been tested for use in ELISA and Western Blot. Expect a band approximately ~125kDa using positive control lysates over-expressing ASAP1, or other specific lysates or tissues. Although not tested, this antibody could be useful in I
Western Blot of Rabbit anti-ASAP1 antibody. Lane 1: ASAP1 transfected U118 lysate. Load: 35 µg per lane. Primary antibody: ASAP1 antibody at 1:1000 for overnight at 4C. Secondary antibody: rabbit secondary HRP antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Block: BlockOut (p/n MB-073) overnight at 4C. Predicted/Observed size: 125 kda for ASAP1.
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