STAT2 pS734 antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to serine 734 a region near the C-terminus of human STAT2 protein.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-STAT2 pS734 antibody, phosphorylated STAT2 antibody, STAT-2, STAT 2, Signal transducer and activator of transcription 2, p113
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5,000, WB: 1:1,000
Anwendungsbeschreibung:
Anti-STAT2 pS734 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 97.9 kDa in size corresponding to STAT2pS734 protein by western blotting
Western Blot of Rabbit anti-STAT2pS734 antibody. Lane 1: U6A cells (STAT2 deficient) untreated. Lane 2: U6A cells reconstituted with STAT2 untreated. Lane 3: U6A cells, STAT2 treated with IFNalpha (1h). Lane 4: U6A cells, STAT2 + IFNalpha (4h). Lane 5: U6A cells, STAT2 + IFNalpha (8h). Lane 6: U6A cells reconstituted with S734A-STAT2 mutant untreated. Lane 7: U6A cells, S734A-STAT2 treated with IFNalpha (1h). Lane 8: U6A cells, S734A-STAT2 + IFNalpha (4h). Lane 9: U6A cells, S734A-STAT2 + IFNalpha (8h). Load: 20 µg per lane. Primary antibody: STAT2 pS734 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 97.9 kDa, ~110 kDa for STAT2pS734. Other band(s): Non specific 100kDa, STAT2, GAPDH loading control.
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