Histone H3 K4me3 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Histone H3 K4me3 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-I59
• Hersteller Artikelnummer: 600-401-I59
• Alternativnummer: ROC-600-401-I59
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ChIP, DOT, IF, WB
• Spezies Reaktivität: C. elegans, Human, Mouse
• Immunogen: Histone H3 [Trimethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 4 of human Histone H3.2.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-Histone H3 trimethyl Lys4 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K4me3

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.70 mg/ml by UV absorbance at 280 nm
• NCBI: 126961
• UniProt: Q71DI3
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ChIP: 2-5 µg/million cells, IHC: 1:500, IF Microscopy: 1:500, WB: 1:500
• Anwendungsbeschreibung: Anti-Histone H3 [Trimethyl Lys4] antibody is tested in Western Blot, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. This antibody is useful for Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user.

Bilder

Rockland Immunochemicals is manufacturing Epi-Plus(TM) antibody in collaboration with Novus Biologicals.

Western Blot of Rabbit Anti-Histone H3 K4/me3 Antibody. Lane 1: C. elegans embryo nuclear extract. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys4] at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Immunofluorescence of Rabbit Anti-Histone H3 K4/me3 Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Trimethyl Lys4] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Trimethyl Lys4] is nuclear and chromosomal. Staining: Histone H3 K4me3 is expressed green and the nuclei are counterstained blue with DAPI.

Chromatin Immunoprecipitaion of Rabbit Anti-Histone H3 K4/me3 Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 K4/me3 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Western Blot of Rabbit Anti-Histone H3 K4/me3 Antibody. Lane 1: HeLa histone preps. Lane 2. NIH-3T3 prep lystates. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys4] at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Dot Blot of Rabbit Histone H3 K4me3 Antibody. Lane 1: K4. Lane 2: K4-KMe1. Lane 3: K4-KMe2. Lane 4: K4-KMe3. Lane 5: K23-KMe3. Load: 1, 3, and 10 µg of peptide. Primary antibody: Histone H3 [Trimethyl Lys4] antibody at 1:1000 for 45 min at 4C. Secondary antibody: Dylight(TM)488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.