RON Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: RON Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-K07
• Hersteller Artikelnummer: 600-401-K07
• Alternativnummer: ROC-600-401-K07
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Anti-RON Antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1360 of human RON protein.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-RON antibody, Macrophage-stimulating protein receptor, MSP receptor, Protein-tyrosine kinase 8, p185-Ron, PTK8, RON, c-met-related tyrosine kinase, CD136

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.06 mg/mL by UV absorbance at 280 nm
• NCBI: 4486
• UniProt: Q04912
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000 - 1:10,000, WB: 1:500-1:2000
• Anwendungsbeschreibung: Anti-RON Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 152.3 kDa corresponding to full length protein by western blotting in the appro

Bilder

Western Blot of Rabbit anti-RON antibody. Left Blot: Phospho RON rProtein (cytoplasmic domain). Right Blot: Phospho RON rProtein (cytoplasmic domain) incubated with RONpY1360 immunizing peptide. Load: 0.05 µg per lane. Primary antibody: RON antibody at 1µg for overnight at 4C. Secondary antibody: HRP Goat anti-rabbit IgG secondary antibody at 1:40,000 for 45 min at RT. Block: MB-070 Fluorescent blocking buffer overnight at 4C. Predicted/Observed size: 152 kDa (full length)/ ~75 kDa (cytoplasmic domain) RON. Other band(s): none.