Anti-Gata1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to mouse Gata1 peptide.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Mouse
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:15,000 - 1:75,000, WB: 1:500 - 1:2,000
Anwendungsbeschreibung:
Anti-Gata1 Antibody has been tested by ELISA and Western Blot. Expect a band approximately 43 kDa in size corresponding to mouse Gata1 by western blotting in the appropriate cell lysate or extract. Researchers should determine optimal titers for applicat
Western Blot of Rabbit Anti-Gata1 antibody. Lane 1: NIH/3T3. Lane 2: mouse brain. Load: 25 µg per lane. Primary antibody: Gata1 antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: MB-070 Fluorescent Blocking Buffer used overnight at 4C. Predicted/Observed size: 42.7 kDa for Gata1.
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