Anti-p130 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal portion of human Retinoblastoma-like protein 2 conjugated to Keyhole Limpet Hemocyanin (KLH).
Konjugation:
Unconjugated
Alternative Synonym:
Rabbit Anti-p130 Antibody, Rabbit Anti-Retinoblastoma-like protein 2 Antibody, RB Transcriptional Corepressor Like 2, 130 KDa Retinoblastoma-Associated Protein, Retinoblastoma-Related Protein 2, Retinoblastoma-Like Protein 2, Retinoblastoma-Like 2, RBR-2
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000 - 1:50,000, WB: 1 µg/ml
Anwendungsbeschreibung:
Anti-p130 Antibody has been tested in ELISA and WB. Expect bands ~128.4 kDa in western blot using appropriate tissues or lysates. Positive control used: MOLT-4 lysate in western blot.
Western Blot of Rabbit Anti-p130 Antibody. Lane 1: MOLT-4 Lysate (p/n W09-001-GK2) (1µg) [+]. Lane 2: MOLT-4 Lysate (p/n W09-001-GK2) (10µg) [+]. Lane 3: Human Eye Lysate (1µg) [-]. Lane 4: Human Eye Lysate (10µg) [-]. Lane 5: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Primary Antibody: Anti-p130 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:10,000 for 1hr at RT. Blocking Buffer: Universal BlockOut (p/n MB-073). Predicted MW: ~128.4kDa. Observed MW: ~125kDa. Exposure: 15sec.
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