Anti-M13 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of Bacteriophage M13 conjugated to Keyhole Limpet Hemocyanin (KLH).
Konjugation:
Unconjugated
Alternative Synonym:
Tail virion protein G7P, Enterobacteria phage M13, Bacteriophage M13,Coat protein C polypeptide I, Gene 7 protein
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000 - 1:50,000, IF Microscopy: User Optimized, WB: User Optimized
Anwendungsbeschreibung:
Anti-Bacteriophage M13 Antibody has been tested in ELISA. Expect a band at ~3.6 kDa in western blot using appropriate lysates or tissues.
ELISA results of purified polyclonal Rabbit Bacteriophage M13 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Coating Buffer pH9.5, Goat Anti-Rabbit HRP conjugated (p/n 611-103-122) at 1:30,000 and substrate (p/n TMBE-1000).
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