Anti-Akirin2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide near the internal region of the human Akirin2.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5,000, WB: 0.5 µg/mL
Anwendungsbeschreibung:
Anti-Akirin2 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 34 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of Akirin2 antibody. Lane A: Human brain tissue lysate in the absence of blocking peptide. Lane B: Human brain tissue lysate in the presence of blocking peptide. Load: 35 µg per lane. Primary Antibody: Anti-Akirin2 at 0.5 µg/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 22.4 kDa, ~35 kDa for Akirin2.
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