Anti-ASAH2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide near the C-terminus of the human ASAH2.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000 - 1:20,000, WB: 1 - 2 µg/mL
Anwendungsbeschreibung:
Anti-ASAH2 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 86 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of ASAH2 antibody. Lane A: 293 cell lysate at 1 µg/mL. Lane B: 293 cell lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 85.5 kDa, ~75 kDa for ASAH2.
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