GST Antibody Biotin Conjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: GST Antibody Biotin Conjugated, Rabbit, Polyclonal
• Artikelnummer: ROC-600-406-200
• Hersteller Artikelnummer: 600-406-200
• Alternativnummer: ROC-600-406-200
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA
• Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.
• Konjugation: Biotin
• Alternative Synonym: rabbit anti-GST antibody biotin conjugation, biotin conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:80,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-GST Biotin Conjugated Antibody has been tested by ELISA and is suitable for ELISA, western blotting and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.

Bilder

Western blot showing detection of recombinant GST protein (0.25 µg) in lane 2. MW markers are in lane 1. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4C primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc(TM) MP 4000 imaging system (Bio-Rad).