Anti-PhosphoTyrosine was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.
Konjugation:
Biotin
Alternative Synonym:
PhosphoTyrosine (pY)reactive antibody, PhosphoTyrosine (pY) polyclonal antibody, PhosphoTyrosine antibody produced in rabbit
Klonalität:
Polyclonal
Konzentration:
1.0 mg/mL by UV absorbance at 280 nm
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5,000, Flow Cytometry: User Optimized, IHC: 10µg/mL, IP: User Optimized, WB: 1:1,000
Anwendungsbeschreibung:
Anti-PhosphoTyrosine antibody is tested for use in ELISA, immunohistochemistry-P, IP, and western blotting assays. The antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing p
Western Blot of Anti-PhosphoTyrosine (pY) Antibody Biotin Conjugated. Load: SP2/0 myeloma cells activated with FBS 10mins after serum free starvation overnight (Lane A: 12µL or Lane B: 4µL whole cell lysates).Primary Antibody: Anti-PhosphoTyrosine (pY) Antibody Biotin Conjugated. Secondary Antibody: Rabbit Anti-Biotin Peroxidase Conjugated.
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