MYC Epitope Tag Antibody Biotin Conjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: MYC Epitope Tag Antibody Biotin Conjugated, Rabbit, Polyclonal
• Artikelnummer: ROC-600-406-381
• Hersteller Artikelnummer: 600-406-381
• Alternativnummer: ROC-600-406-381
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: DOT, ELISA, WB
• Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
• Konjugation: Biotin
• Alternative Synonym: Rabbit anti-MYC Epitope Tag Biotin Conjugated Antibody, Rabbit anti-c-myc tag Biotin conjugation, Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:100,000 - 1:500,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene. As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope. The antibody recognizes the Myc tag fused either to the amin

Bilder

Western Blot of Biotin Conjugated Rabbit anti-Myc Epitope Tag antibody. Lane 1: 12-Tag Lysate. Lane 2: None. Load: N/A. Primary antibody: None. Secondary antibody: Biotin rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Myc Epitope Tag. Other band(s): Myc splice variants and isoforms.

Anti-Myc epitope tag polyclonal antibody detects Myc-tagged recombinant proteins by western blot. Polyclonal rabbit-anti-Myc epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 ug of recombinant protein containing the Myc epitope tag. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.