HA Epitope Tag Antibody Biotin Conjugated, Rabbit, Polyclonal
Artikelnummer:
ROC-600-406-384
Hersteller Artikelnummer:
600-406-384
Alternativnummer:
ROC-600-406-384
Hersteller:
Rockland Immunochemicals
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA, WB
Immunogen:
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
Konjugation:
Biotin
Alternative Synonym:
Rabbit Anti-HA Epitope Tag Antibody Biotin Conjugated, Rabbit Anti HA Epitope Tag Antibody Biotin Conjugated
Klonalität:
Polyclonal
Konzentration:
1.0 mg/mL by UV absorbance at 280 nm
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Anti-HA is optimally suited for monitoring the expression of HA tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- terminus of targ
Anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by western blot. Polyclonal rabbit-anti-HA epitope tag at a 1:10,000 dilution was used to detect 1.0 ug of recombinant transcription factor protein containing the HA epitope tag. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with Rocklands anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:4000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results
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